A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions
نویسندگان
چکیده
منابع مشابه
A near-infrared BiFC reporter for in vivo imaging of protein-protein interactions.
Studies of protein-protein interactions deep in organs and in whole mammals have been hindered by a lack of genetically encoded fluorescent probes in near-infrared region for which mammalian tissues are the most transparent. We have used a near-infrared fluorescent protein iRFP engineered from a bacterial phytochrome as the template to develop an in vivo split fluorescence complementation probe...
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Protein-protein interaction (PPI) plays a pivotal role in a wide variety of cellular events and physiological functions, such as enzymatic activity, signal transduction, immunological recognition, DNA repair/replication, among others (Valdar and Thornton, 2001). In addition, biological events that regulate proliferation, differentiation, and inflammation are also commonly mediated through PPI (...
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Protein-protein interactions are a hallmark of all essential cellular processes. However, many of these interactions are transient, or energetically weak, preventing their identification and analysis through traditional biochemical methods such as co-immunoprecipitation. In this regard, the genetically encodable fluorescent proteins (GFP, RFP, etc.) and their associated overlapping fluorescence...
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متن کاملMONITORING iN ViVO PROTEIN-PROTEIN INTERACTIONS BY COUPLING BIMOLECULAR FLUORESCENCE COMPLEMENTATION (BIFC) AND FLOW CYTOMETRY
We present a high-throughput approach to study weak protein-protein interactions by coupling bimolecular fluorescent complementation (BiFC) to flow cytometry (FC). Bimolecular Fluorescence Complementation (BIFC) is a method to detect proteinprotein interactions based on the formation of a fluorescent complex by fragments of the yellow fluorescent protein (NYFP and CYFP) brought together by the ...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2013
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2013.06.009